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Título : Listeriolysin S : a bacteriocin from "Listeria monocytogenes" that induces membrane permeabilization in a contact-dependent manner
Autor : Meza Torres, Jazmín.
Lelek, Mickaël.
Quereda Torres, Juan José.
Sachse, Martin.
Manina, Giulia.
Ershov, Dmitry.
Materias: Food poisoning.Bacteriocinas.Listeriolysin S (LLS)Listeriosis.Veterinary microbiology.Microbiología veterinaria.Intoxicación por alimentos.Listeria monocytogenes.Bacteriocins.
Fecha de publicación : 1-oct-2021
Editorial : National Academy of Science
Citación : Meza-Torres, J., Lelek, M., Quereda, J.J., Sachse, M., Manina, G., Ershov, D. et al. (2021). Listeriolysin S : a bacteriocin from "Listeria monocytogenes" that induces membrane permeabilization in a contact-dependent manner. Proceedings of the National Academy of Sciences (PNAS), vol. 118, i. 40 (01 oct.), art. e2108155118. DOI: https://doi.org/10.1073/pnas.2108155118
Resumen : Listeriolysin S (LLS) is a thiazole/oxazole–modified microcin (TOMM) produced by hypervirulent clones of Listeria monocytogenes. LLS targets specific gram-positive bacteria and modulates the host intestinal microbiota composition. To characterize the mechanism of LLS transfer to target bacteria and its bactericidal function, we first investigated its subcellular distribution in LLS-producer bacteria. Using subcellular fractionation assays, transmission electron microscopy, and single-molecule superresolution microscopy,we identified that LLS remains associated with the bacterial cell membrane and cytoplasm and is not secreted to the bacterial extracellular space. Only living LLS-producer bacteria (and not purified LLS-positive bacterial membranes) display bactericidal activity. Applying transwell coculture systems and microfluidic-coupled microscopy, we determined that LLS requires direct contact between LLS-producer and -target bacteria in order to display bactericidal activity, and thus behaves as a contact-dependent bacteriocin. Contact-dependent exposure to LLS leads to permeabilization/depolarization of the target bacterial cell membrane and adenosine triphosphate (ATP) release. Additionally, we show that lipoteichoic acids (LTAs) can interact with LLS and that LTA decorations influence bacterial susceptibility to LLS. Overall, our results suggest that LLS is a TOMM that displays a contact-dependent inhibition mechanism.
Descripción : Este artículo se encuentra disponible en la siguiente URL: https://www.pnas.org/doi/epdf/10.1073/pnas.2108155118
En este artículo de investigación también participan: Jean-Yves Tinevez, Lilliana Radoshevich, Claire Maudet, Thibault Chaze, Quentin Giai Gianetto, Mariette Matondo, Marc Lecuit, Isabelle Martin-Verstraete, Christophe Zimmer, Hélène Bierne, Olivier Dussurget, Pascale Cossart y Javier Pizarro-Cerdá.
Idioma: en
es
URI : http://hdl.handle.net/10637/13710
Derechos: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
ISSN : 0027-8424
1091-6490 (Electrónico)
Aparece en las colecciones: Dpto. Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos

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